Browsing by Author "Birch, RG"

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    Development of a biological control against sugarcane poor root syndrome
    (BSES, 1987) Birch, RG
    The overall objective of the project was to search for and develop an economically effective biocontrol against sugarcane poor root syndrome (PRS). The disease was known to be caused by two pathogenic fungi: Pythium graminicola and an unnamed oomycete causing flaccid root rot. The seriousness of this newly recognised disease, and the dearth of economic controls warranted the effort necessary for such a search.
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    Molecular tagging of economically important genes and promoters in sugarcane
    (1992) Birch, RG
    Project Objectives : The overall aim of this project was to develop techniques for the isolation of useful gene control sequences (promoters) in sugarcane. Early in the project we also undertook preliminary studies to lay some groundwork for techniques to tag and isolate useful genes in sugarcane. Specific objectives were: I. Develop techniques for the isolation of specific sugarcane gene control sequences. This led us to evaluate the applicability of differential cDNA hybridisation to recover tissue specific genes from sugarcane, followed by genomic library screening to recover corresponding tissue-specific promoters. 2. Prepare genetic constructs for transfer into sugarcane to assist identification and isolation of useful genes and promoters in sugarcane. This led us to test the effectiveness of a range of well characterised foreign promoters and novel artificial promoters in sugarcane. 3. Probe the sugarcane genome for endogenous transposable elements homologous to those isolated from other Oramineae, to help evaluate the potential for transposon tagging of useful sugarcane genes. All project objectives were achieved.