A sugarcane tissue culture system for mass propagation and transformation : SRDC final report BSS242
Abstract
The core research strategy we conceived was to develop the initial observation of direct
shoot regeneration in sugarcane leaf tissues to a generic and efficient plant production
technology for sugarcane and probably for other major monocot crops, and then use that
system to develop a novel method for sugarcane transformation.
The main objectives of this project were:
1. Protect identified IP by patent;
2. Develop an efficient direct plant regeneration system for sugarcane and determine
clonal stability of plants produced by this method (now referred to as the SmartSett®
system);
3. Develop microprojectile and/or Agrobacterium-mediated transformation systems
based on SmartSett®;
4. Establish the utility of SmartSett® to other monocot crops;
5. Technology transfer to other groups.
These objectives were met.