Enhancement of the sensitivity of gene probes for viral diseases of sugarcane by the polymerase chain reaction : SRDC final report BS48S
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The sensivity of gene probes for two important viral pathogens of sugarcane, sugarcane mosaic virus (SCMV) and Fiji disease virus (FDV), has been significantly enhanced by a version of the polymerase chain reaction (PCR). This version, known as RT-PCR, involves a reverse transcription (RT) step to synthesise DNA from RNA template prior to the PCR amplication. RT-PCR increased the level of sensitivity of detection by 10(3)-fold for SCMV and by up to 10(6)-fold for FDV. The RT-PCR conditions for routine detection of SCMV and FDV in sugarcane tissues were determined, but the glasshouse trial was not completed.