Show simple item record

OrganisationInternational Consortium for Sugarcane Biotechnology
AuthorD'Hont, A
Date Accessioned2013-12-12
Date Available2013-12-12
AbstractA major gene for rust resistance has been identified in cultivar R570 (Daugrois et ai, 19%). Rust disease is caused by the fungus Puccinia malenocephala Syd P. Syd and is distributed worldwide. Inheritance of resistance to rust was investigated in the self progeny of cultivar R570, which was also used to build the RFLP genetic map of this cultivar (Grivet et ai, 1996). Resistance was evaluated through both field and controled greenhouse trials. The results demonstrated that the field scores are very well correlated between plant cane and first ratoon and in very good agreement with the greenhouse artificial inoculation tests. A cle;r-cut 3 (resistance) : 1 (susceptible) segregation indicative of a dominant resistance gene was observed. RFLP mapping indicated that this gene was linked at approximately 10 cM to a marker revealed by the cDNA clone CDSR 29. This marker was not linked to any of the cosegregation groups of the R570 map (Grivet et ai, 19%). This gene is the first major gene identified in sugarcane. ICSB was thus interested in using it to test the feasability of map-based cloning in the complex aneuploid polyploid that sugarcane is. For this purpose the BAC library developed by RWing and co-workers was constructed using cultivar R570 (Tomkins et ai, 1999). The first step for map-based cloning is to develop a fine map in the vicinity of the gene; this was the objective of the present project. For this purpose, we developed and characterized for rust resistance large selfed progenies of R570 and we followed two strategies to find markers closely surrounding the rust resistance gene ; one is based on the synteny and colinearity demonstrated between Graminae species and the second is based on Bulked Segregant Analysis (BSA) with AFLP markers. We also tested the pathogenicity variability of the fungus.
Part of SeriesInternal Report; 1999 ICB004
SubjectRust resistance
SubjectMap-based cloning
SubjectSegregant analysis (BSA)
SubjectPlant breeding
TitleFine mapping of major rust resistance gene in cultivar R570 with a view towards it closing through map-bases chromosome walking

Files in this item


This item appears in the following Collection(s)

  • Varieties, plant breeding and release [124]
    Research outcomes: Comprehensive and efficient variety breeding, selection and release programs responding to yield expectations, environmental constraints, resource scarcity and regional preferences. Faster varietal adoption using advanced methods for bulking, distribution and planting.

Show simple item record