Show simple item record

AuthorSmith, GR
Date Accessioned2012-11-13
Date Available2012-11-13
Issued1994
Identifierhttp://hdl.handle.net/11079/711
AbstractTransgenic sugarcane plants, which expressed the coat protein of SCMV at a very low level, were produced by micoprojectile bombardment of sugarcane meristems. Transgenic plants expressing the luciferase (Luc) and B-glucuronidase (GUS) reporter genes were also produced. A paper describing regeneration of plantlets expressing the reporter gene GUS (Plant Cell Reports 12:343-346) was the first report of the use of sugar meristem tissues as a transformation target, and the second report of transgenic sugarcane plants. The level of expression of the coat protein gene in the regenerated plantlets was very low, possibly due to chimaerism, ie. mixtures of transformed and non-transformed cells in the same tissues. We have established that sugarcane meristems are a useful target for microprojectile transformation of sugarcane, although more research is necessary before this target can be routinely used for sugarcane genetic engineering.
Languageen
Part of SeriesBSES Internal Report; 1994 No 687 Report SD94005
SubjectSugarcane
SubjectTransgenic plants
SubjectGus
SubjectScmv
SubjectVarieties
SubjectPlant breeding
TitleThe production of transgenic sugarcane plants : SRDC final report BS44S


Files in this item

Thumbnail

This item appears in the following Collection(s)

  • Varieties, plant breeding and release [124]
    Research outcomes: Comprehensive and efficient variety breeding, selection and release programs responding to yield expectations, environmental constraints, resource scarcity and regional preferences. Faster varietal adoption using advanced methods for bulking, distribution and planting.

Show simple item record