A sugarcane tissue culture system for mass propagation and transformation : SRDC final report BSS242

Abstract

The core research strategy we conceived was to develop the initial observation of direct shoot regeneration in sugarcane leaf tissues to a generic and efficient plant production technology for sugarcane and probably for other major monocot crops, and then use that system to develop a novel method for sugarcane transformation. The main objectives of this project were: 1. Protect identified IP by patent; 2. Develop an efficient direct plant regeneration system for sugarcane and determine clonal stability of plants produced by this method (now referred to as the SmartSett® system); 3. Develop microprojectile and/or Agrobacterium-mediated transformation systems based on SmartSett®; 4. Establish the utility of SmartSett® to other monocot crops; 5. Technology transfer to other groups. These objectives were met.