Further characterisation of pathogenicity genes from Leifsonia xyli subsp. xyli, causal organism of ratoon stunting disease : SRDC final project report BSS203

Abstract

In a previous SRDC/CRCTPP/BSES-funded project, a gene from the sugarcane pathogen Leifsonia xyli subsp. xyli (Lxx) was tagged by transposon mutagenesis, cloned and sequenced, and, based on DNA homology searches, was found to encode what appeared to be a putative integral membrane protein (PIMP). To try to better understand the role of a PIMP in Lxx pathogenicity on sugarcane, this project was undertaken to sequence the flanking regions to determine if this gene was part of an operon or a pathogenicity island.Two overlapping cosmids, both with homology to the flanking region of the transposon in mutant Lxx