Maintaining access to foreign germplasm by developing methods to detect unidentified viruses in sugarcane in quarantine : SRDC final report BS116S

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dc.contributor.authorBraithwaite, KS
dc.contributor.authorSmith, GR
dc.date.accessioned2012-11-13T02:29:00Z
dc.date.available2012-11-13T02:29:00Z
dc.date.issued1998
dc.description.abstractFour types of gneeric tests for detecting unidentified viruses in plants were trialed for their suitability for sugarcane. The tests were; double stranded RNA (dsRNA) analysis, sap inoculation of indicator plants, sap examination by electron microscopy and polymerisation chain reaction (PCR) amplification using 'group-specific' primers. The aim of this project was to develop and implement general diagnostic tests for unidentified viruses in sugarcane germplasm held in quarantine. The availability of these general tests has provided an opportunity to significantly improve the security of sugarcane quarantine.
dc.identifier.urihttp://hdl.handle.net/11079/843
dc.language.isoenen
dc.relation.ispartofseriesBSES Internal Report; 1998 No 899 Report SD98003; SRDC BSS116
dc.subjectDiagnostics
dc.subjectGeneric test
dc.subjectVirus detection
dc.subjectDouble stranded RNA (dsRNA) analysis
dc.subjectSap innoculation of indicator plants
dc.subjectQuarantine manual
dc.subjectVarietiesen
dc.subjectPlant breedingen
dc.titleMaintaining access to foreign germplasm by developing methods to detect unidentified viruses in sugarcane in quarantine : SRDC final report BS116S

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