Regeneration of sugarcane plants from protoplasts

Abstract

This study aimed to develop a protoplast regeneration system for sugarcane so that gene transfer techniques could be applied for cultivar improvement. Published techniques for embryogenic callus culture, cell suspension culture, protoplast culture and plant regeneration in sugarcane were developed and tested using only a few cultivars. These techniques were further developed, evaluated and adapted for application to a range of commercial sugarcane cultivars.