Browsing by Author "Grof, C"

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    A sugarcane tissue culture system for mass propagation and transformation : SRDC final report BSS242
    (2004) Lakshmanan, P; Grof, C; Geijskes, R
    The core research strategy we conceived was to develop the initial observation of direct shoot regeneration in sugarcane leaf tissues to a generic and efficient plant production technology for sugarcane and probably for other major monocot crops, and then use that system to develop a novel method for sugarcane transformation. The main objectives of this project were: 1. Protect identified IP by patent; 2. Develop an efficient direct plant regeneration system for sugarcane and determine clonal stability of plants produced by this method (now referred to as the SmartSett® system); 3. Develop microprojectile and/or Agrobacterium-mediated transformation systems based on SmartSett®; 4. Establish the utility of SmartSett® to other monocot crops; 5. Technology transfer to other groups. These objectives were met.
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    Building strategic research capacity for the sugar industry aimed at overcoming physiological, biochemical and environmental constraints to cane growth, breeding and sucrose yield
    (1999) Grof, C; Campbell, J
    Using the CSIRO Controlled Environment Facility, the discrete effects on the rate and extent of sucrose accumulation in sugarcane of four significant environmental parameters (daily light integral, radiation levels, temperature and humidity) have been investigated. These experiments have attempted to address some existing industry problems and have provided some basic understanding of sugarcane physiology, a platform from which to launch a more detailed investigation of the metabolic processes that control the rate and final level of sucrose accumUlation.
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    The transfer of high CCS traits from wild relatives to sugarcane using biochemical markers
    (2003) Grof, C; Manners, J
    Over the last 40 years of sugarcane breeding in Australia there have been significant improvements in cane yield but little to no improvement in commercial cane sugar (CCS). It has been hypothesised that this lack of gain is due to the narrow genetic base of current breeding programs and has provided the impetus to examine new sources of germplasm that may provide desirable traits. Broadening the genetic base through the introgression of new Saccharum germplasm could allow the incorporation of 'new' genes for CCS into commercial sugarcane and result in increased CCS. However carefully targeted approaches are required to identify favourable genetic components of value from available new germplasm for introgression.
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    Transformation of sugarcane using Agrobacterium tumefaciens : final report on CTA027
    (2000) Grof, C; Elliott, A
    A method for Agrobacterium-mediated transformation of sugarcane tissues has been developed in this project. This method offers an alternative technique for the introduction of transgenes into sugarcane. In general, Agrobacterium is viewed as the method of choice for the range of plant species where both Agrobacterium and microprojectile-mediated techniques have been applied successfully. A number of transgenic sugarcane plants have been produced in this project using different Agrobacterium vectors, different selection stnitegies, different techniques and the protocol has been published in an international journal. The applicability of the technique to sugarcane cvs Q117, NCo-310, QI24 and QI55 has also been demonstrated. Many experiments were performed to optimise the Agrobacterium transformation methodology for sugarcane with parameters having a significant effect on transformation efficiency being identified. A selection system based on herbicide resistance was implemented to select for the growth of transgenic cells. The GFP system was further developed and facilitated the rapid production of transgenic cell lines and the scoring of transformation experiments. Collaborative work with the BSES has led to the development of new techniques and opportunities, which should result in a very useful industry outcome. Although the development of an Agrobacterium-mediated transformation technique for sugarcane is described here, the real value of the technique will not be realized until the integration patterns, level of gene expression and level of somaclonal variation are determined in the transgenic plants produced.