Browsing by Author "Taylor, PWJ"
Now showing 1 - 9 of 9
- Results Per Page
- Sort Options
Item Development of a fungicide spray schedule to determine the effect of sugarcane rust (Puccinia melanocephala) on yield(BSES, 1984) Taylor, PWJ; Croft, BJ; Ryan, CCThis study reports on work carried out to develop a fungicide spray program which is suitable for yield loss assessment investigations. Aspects examined include the efficacy of various concentrations of chlorothalonil and oxycarboxin on rust, the frequency of application of one concentration of chlorothalonil or oxycarboxin on level of infection, and arrangement of spray nozzles to give good coverage of fungicides.Item Epidemiology and control of sugarcane mosaic virus in the Isis district : SRC final report UQ3S(BSES, 1992) Teakle, DS; Taylor, PWJ; Allsopp, PGObjectives:Item Ratoon stunting disease (RSD) host-pathogen relationships in sugarcane : SRDC final report BS99S(BSES, 1997) Brumbley; Birch, R; Croft, BJ; Taylor, PWJ; Petrasovits, LAClavibacter xyli subsp. xyli (Cxx) is a gram +, fastidious, slow growing bacterium that causes ratoon stunting disease (RSD) of sugarcane. Because of its fastidious nature, slow growth, and poor symptom expression, it has traditionally been put into the 'too hard basket' by molecular plant pathologists. Systems for transformationa nd transposon-based mutagenesis of Cxx have been successfully established. In addition, a very sensitive polymerase chain reaction (PCR) based detection system was developed for Cxx so that as few as 100 cells per reaction can be detected in the xylem extracts from Cxx infected plants.Item Regeneration of sugarcane plants from protoplasts(1991) Taylor, PWJThis study aimed to develop a protoplast regeneration system for sugarcane so that gene transfer techniques could be applied for cultivar improvement. Published techniques for embryogenic callus culture, cell suspension culture, protoplast culture and plant regeneration in sugarcane were developed and tested using only a few cultivars. These techniques were further developed, evaluated and adapted for application to a range of commercial sugarcane cultivars.Item Regeneration of sugarcane plants from protoplasts and application of genetic transformation systems to sugarcane(1986) Taylor, PWJCurrent gene transfer systems developed for modifying plants include direct gene transfer to protoplasts and Agrobacterium mediated gene transfer. These studies have important practical implications for application of genetic engineering technology to crop improvement, initially in sugarcane, and subsequently in other graminaceous crops for which sugarcane may serve as a model.Item Studies into races of sugarcane rust(BSES, 1984) Taylor, PWJA technique was developed for testing for physiological races of sugarcane rust. A range of cultivars were inoculated with a single spore isolate of Puccinia melanocephala by discharging 3 mg of urediospores into a spore settling tower by compressed air at 200 kPa pressure for one second with a settling time of ten minutes. A range of reaction types were obtained using this method. These reaction types were similar to reactions on naturally infected field grown plants. Components of stable resistance such as generation time, uredia number per mm2 and uredia diameter were also evaluated. The more resistant cultivars had fewer uredia per mm2 compared to the susceptible cultivars. No difference was recorded in generation times and size of uredia. The age of the leaf inoculated (between 24 and 75 days) did not have any apparent effect on host reaction type, generation time, uredia number and uredia size.Item The effect of sugarcane rust (Puccinia melanocephala) on yield(BSES, 1985) Taylor, PWJ; Croft, BJ; Ryan, CCThis report details the results obtained from two trials to assess yeild loss caused by sugarcane rust and also outlines the epiphytotiology of the rust disease.