Completed projects and reports

Now showing 1 - 6 of 6

Improving the Plant breeding selection system for Fiji Disease Resistance : SRDC final report BSS255
(2005) Croft, BJ; Ridley, AW; Dhileepan, K; James, AP
Fiji leaf gall (FLG) has caused major epidemics in the Australian sugar industry in the past. All new cultivars of sugarcane released in areas affected by FLG should be resistant to the disease. New cultivars have been tested for resistance in field trials in the past but the field trials are unreliable with only a third of trials in the last 28 years giving reliable ratings. The aim of this project was to develop a method of rating clones for resistance to FLG that would be accurate, reliable and practical to implement in the BSES-CSIRO variety improvement program.
Characterisation and maintenance of the Australian sugarcane mapping populations
(BSES, 2003) McIntyre, L
There were two major aims to this project. The first was to identify markers linked to major diseases of sugarcane that were difficult and expensive to select for. The second objective was to determine the cross-transferability of markers by testing the association between markers and traits in other germplasm. Both of these aims have been successfully addressed. Fiji Disease virus (FDV) and Pachymetra are two major diseases of sugarcane. Both require laborious and expensive screening in the field or glasshouse and consequently, only limited numbers of clones can be screened. Thus, both of these traits are candidates for molecular markers. Successful identification of molecular markers associated with these diseases could enable indirect selection for resistance to be undertaken, in the absence of screening, or with reduced screening, for the diseases themselves. Two populations, Q117 x 74C42 and Q96 x Q178, were developed by Dr Nils Berding (BSS038) and each contained more than 200 progeny. Both populations had been previously scored for numerous sugar-related and agronomic traits and marker-trait associations (QTLs) identified for all traits in the Q117 x 74C42 population. One objective in this project was to see if QTLs identified in Q117 x 74C42 would detect the same traits in the Q96 x Q178 population. Unfortunately, in the process of mapping in this second population, it was discovered that the population was a mixture of several very small, different populations. This project objective could not be completed as planned, but was modified, as discussed below. In addition to the approximately 300 markers already scored on the Q117 x 74C42 population (CTA024), a further 1100 amplified fragment length polymorphism markers (AFLPs), simple sequence repeat (SSR) markers and resistance gene analogue (RGAs) were scored. The first two types were used as they are PCR-based, reliable, easy to generate, and the type of markers currently being used in other sugarcane maps. RGAs are potential candidate genes for disease resistance. Unfortunately, during the amalgamation of the marker information, it became apparent that the replanted progeny clones had become “renumbered” in the field. It was thus not possible to combine the two data sets, and consequently, new maps were developed for the Q117 x 74C42 population using just the AFLP, RGA and SSR data. The 1100 markers were used to develop two parental maps. The Q117 map contains 407 markers in 75 linkage groups, while the 74C42 map contains 447 markers in 84 linkage groups.
Report by the consultant phytopathologist on the mission to investigate the Fiji disease situation in Madagascar
(BSES, 1991) Egan, BT
Presents findings, conclusion and recommendations of the mission to investigate the Fiji disease situation in Madagascar.
Inheritance of Fiji disease resistance
(BSES, 1981) Hogarth, DM; Ryan, CC
Various projects have been conducted in recent years to study the inheritance of resistance to Fiji disease. In this report the results of these studies are presented and the implications for the plant breeding programme discussed.
Acquisition of Fiji disease virus by Perkinsiella saccharicida kirk and observations on its instars
(BSES, 1984) Croft, BJ; Ryan, CC
The first, second and third instars of Perkinsiella saccharicida were the only stages which transmitted Fiji disease virus to healthy Q70 plants after a 48 hour access feed on diseased plants. A key to distinguish the developmental stages of P.saccharicida was developed with the main distinguishing features being eye colour, and the shape, length and relationship, one to the other, of the fore and hind wing pads.
The production and evaluation of gene probes for diagnosis of sugarcane mosaic virus and Fiji disease virus (Final report SRDC Project BS10S)
(BSES, 1991) Smith, GR; Leonard, GJ
Specific and sensitive cDNA probes have been developed for detection of both FDV and SCMV in diseased sugarcane plants. Results from glasshouse evaluation indicate that FDV probes are not sensitive enough to be used for the detection of the virus in asymptomatic diseased plants. Results are not yet available from the SCMV probe glasshouse trial.

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