Completed projects and reports

Permanent URI for this communityhttp://elibrary2.sugarresearch.com.au/handle/11079/13840

Sugar Research Australia, Sugar Research Development Corporation and BSES reports from completed research projects and papers.

Browse

Filters

Advanced Search

Filter by

Settings

Has files: YesSubject: search.filters.subject.Ratoon stunting disease

Search Results

Now showing 1 - 5 of 5
  • Thumbnail Image
    Item
    Support for an ARC project to investigate genetic diversity of Leifsonia xyli subsp. xyli , causa organism of ratoon stunting disease : SRDC final report BSS239
    (BSES, 2004) Brumbley, SM
    The primary aim of this research was to genetically characterise populations of Leifsonia xyli subsp. xyli (Lxx), causal agent of ratoon stunting disease (RSD) of sugarcane.A collection of 108 isolates of Lxx collected from nine countries was investigated using various molecular techniques. These included randomly amplified polymorphic DNA (RAPD) analysis, restriction analysis of amplified 16SrRNA genes, single stranded conformational polymorphism (SSCP) analysis of the 16S-23S intergenic spacer (ITS), and sequence analysis of the 16S rRNA and ITS. No variation was detected using any technique employed, indicating a high degree of relatedness between the isolates. The apparent absence of variation in strains of Lxx holds promise for both RSD-resistance breeding work and genetic engineering of resistance into elite sugarcane cultivars.
  • Thumbnail Image
    Item
    Further characterisation of pathogenicity genes from Leifsonia xyli subsp. xyli, causal organism of ratoon stunting disease : SRDC final project report BSS203
    (BSES, 2002) Brumbley, SM
    In a previous SRDC/CRCTPP/BSES-funded project, a gene from the sugarcane pathogen Leifsonia xyli subsp. xyli (Lxx) was tagged by transposon mutagenesis, cloned and sequenced, and, based on DNA homology searches, was found to encode what appeared to be a putative integral membrane protein (PIMP). To try to better understand the role of a PIMP in Lxx pathogenicity on sugarcane, this project was undertaken to sequence the flanking regions to determine if this gene was part of an operon or a pathogenicity island.Two overlapping cosmids, both with homology to the flanking region of the transposon in mutant Lxx
  • Thumbnail Image
    Item
    Ratoon stunting disease (RSD) host-pathogen relationships in sugarcane : SRDC final report BS99S
    (BSES, 1997) Brumbley; Birch, R; Croft, BJ; Taylor, PWJ; Petrasovits, LA
    Clavibacter xyli subsp. xyli (Cxx) is a gram +, fastidious, slow growing bacterium that causes ratoon stunting disease (RSD) of sugarcane. Because of its fastidious nature, slow growth, and poor symptom expression, it has traditionally been put into the 'too hard basket' by molecular plant pathologists. Systems for transformationa nd transposon-based mutagenesis of Cxx have been successfully established. In addition, a very sensitive polymerase chain reaction (PCR) based detection system was developed for Cxx so that as few as 100 cells per reaction can be detected in the xylem extracts from Cxx infected plants.
  • Thumbnail Image
    Item
    Economic analysis of RSD control strategies : final report SRDC project BS101S
    (BSES, 1995) Croft, BJ; Page, JR; Bull, JK; Beattie, RN
    The aim of this study was to compare the economics of the existing RSD control strategy in Australia of disease-free seed and disinfecting harvesters with breeding cultivars resistant to RSD. Analysis presented in this paper show that an effective disease-free seed scheme is economically the best RSD control strategy. Cultivar resistance would only be attractive if disease-free seed ceased to be a viable option.
  • Thumbnail Image
    Item
    A method for the rapid identification of ratoon stunting disease in sugar cane
    (BSES, 1977) Steindl, DRL
    Work with the electron microscope established a slender coryneform - type bacterium is constantly present in the sap of known ratoon stunting diseased plants and is absent from healthy plants. Purpose of project was to determine whether a reliable method of diagnosing doubtful cases of the disease could be based on the fundings of such organisms in the extracted sap, either by election or phase - contrast microscopy.